|Calculated MW||51184 Da|
|Homology||Mouse- identical; human - 17/19 amino acidresidues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit alpha-6, GABA(A) receptor subunit alpha-6, Gabra6, Gabra-6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide QLEDEGNFYSENVSRILDN(C), corresponding to amino acidֲ residues 20-37 of rat GABA(A) ־±6 Receptor (Accessionֲ P30191). Extracellullar, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1 The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and the metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2 The GABA(A) R, is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABA(A) receptor, in most cases, consists of 2α, 2β and 1γ subunit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABA(A)R. A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A) α6 along with β2, α1 and γ2, in alcohol related phenotypes.3 In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A) α6 was reported.4 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α6 Receptor. The Anti-GABA(A) α6 Receptor (extracellular) antibody (#AG1276) can be used for western blot analysis and Immunohistochemical applications. It has been designed to recognize GABA(A) α6 Receptor from rat, mouse and human samples.
References 1. Owens,D.F. and Kriegstein, A.R. (2002) Nature Rev. Neurosci. 3, 715. 2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387. 3. Loh, E.W and Ball, D. (2000) Neurochem. Int. 37, 413. 4. Surendran, S. et al. (2003) Mol. Gen. Met. 80, 74.
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