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GABA (A) alpha3 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GABA (A) alpha3 Receptor (extracellular) Antibody AG1277-025
    Western blot analysis of rat brain membranes:
    1. Anti-GABA(A) α3 Receptor (extracellular) antibody (#AG1277), (1:200).
    2. Anti-GABA(A) α3 Receptor (extracellular) antibody preincubated with the control peptide antigen.
  • ICC - GABA (A) alpha3 Receptor (extracellular) Antibody AG1277-025
    Expression of GABA(A) α3 Receptor in rat insulinoma cell line Immunocytochemical staining of intact living rat RIN-m cells using Anti- GABA(A) α3 Receptor (extracellular) antibody (#AG1277), (1:50) followed by goat anti-rabbit-AlexaFluor-550 secondary antibody. Extracellular staining (red) merged with live view of the cells.
  • IHC - GABA (A) alpha3 Receptor (extracellular) Antibody AG1277-025
    Expression of GABA(A) α3 Receptor in rat cerebellum
    Immunohistochemical staining of rat cerebellum using Anti-GABA(A) α3 Receptor (extracellular) (#AG1277), (green), (1:100). GABA(A) α3 Receptor is localized to the molecular layer (asterisk) and a portion of the Purkinje cell body (arrows), which is outlined by axonal staining using mouse anti-neurofilament 200 (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P34903
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 55165 Da
Homology Rat, mouse - identical; bovine - 14/15 amino acid residues identical.
Additional Information
Gene ID 2556
Other Names Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABRA3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABA (A) ־±3 Receptor (Accession P34903). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Mouse brain lysates (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Mouse brain lysates (1:500) (see Zhou, C. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Transfected COS-7 cells (1 µg antibody/140 µg lysate) (see Zhou, C. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse brain sections (1:200) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Mouse brain sections (1:500) (see Zhou, C. et al. (2013) in Product Citations). - Rat brain sections (1:250) (see Park, H.J. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Research Areas
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The neurotransmitter GABA (γ-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2   There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.1,2   GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore. Eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ.Six α subunits isoforms (α1-α6) have been shown to exist in mammals. In most cases, the native GABAA receptors consist of 2α, 2β, and 1γ subunits.  The α3-subunit is highly expressed during development (along with α2 and α5) and then declines in adulthood, where the α1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.3


References 1. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. 3. Fuchs, K. and Celepirovic, N.. (2002) J. Neurochem. 82, 1512.

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Cat# AG1277-025
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