|Reactivity||Human, Mouse, Rat|
|Calculated MW||55165 Da|
|Homology||Rat, mouse - identical; bovine - 14/15 amino acid residues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABRA3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABA (A) ־±3 Receptor (Accession P34903). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse brain lysates (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Mouse brain lysates (1:500) (see Zhou, C. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Transfected COS-7 cells (1 µg antibody/140 µg lysate) (see Zhou, C. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse brain sections (1:200) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Mouse brain sections (1:500) (see Zhou, C. et al. (2013) in Product Citations). - Rat brain sections (1:250) (see Park, H.J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The neurotransmitter GABA (γ-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2 There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.1,2 GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore. Eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunits isoforms (α1-α6) have been shown to exist in mammals. In most cases, the native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α3-subunit is highly expressed during development (along with α2 and α5) and then declines in adulthood, where the α1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.3
References 1. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. 3. Fuchs, K. and Celepirovic, N.. (2002) J. Neurochem. 82, 1512.
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