|Reactivity||Human, Mouse, Rat|
|Calculated MW||51182 Da|
|Homology||Mouse, human, bovine - identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2, Gabra2, Gabra-2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)TPEPNKKPENKPA, corresponding to amino acid residues 393-405 of rat GABA (A) ־±2 Receptorֲ (accession number P23576). 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1 The GABA transmitter interacts with two major types of receptors: ionotropic GABAA (GABAAR) and the metabotropic GABAB) receptors. The GABAAR belong to the ligand gated ion channel superfamily.2 The GABAAR, is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ submit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABAAR. The α2-subunit mRNA is strongly expressed only in the hippocampus and the hypothalamus. It occurs very early in rat brain development and declines in adulthood. The failure to complete the normal transition between the a-subunits that are highly expressed in early development (α2, α3, α5) to those expressed in adulthood (α1) might play a major role in the development of temporal lobe epilepsy.3
References 1. Owens, D.F. and Kriegstein, A.R. (2002) Nature Rev. Neurosci. 3, 715. 2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387. 3. Fuchs, K. et al. (2002) J. Neurochem. 82, 151.
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