|Application ||WB, LCI|
|Calculated MW||51754 Da|
|Homology||Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, Gabra1, Gabra-1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABA (A) ־±1 Receptor (Accession P62813). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse brain lysates (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Rat brain lysates (see Woo, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse brain sections (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Rat brain sections (1:250) (see Park, H.J. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat adrenal medullary cells (1:100) (see Matsuoka, H. et al. (2008) in Product Citations). Live cell imaging (LCI): - Rat living hippocampal neurons (1:400) (see Pribiag, H. and Stellwagen, D. (2013) in Product Citations). - Rat living hippocampal neurons (1:500) (see Chaumont, S. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1 The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2 GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands. Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5
References 1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715. 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. 3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. 4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057. 5. Fuchs, K. and Celepirovic, N. (2002) J. Neurochem. 82, 1512.
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