Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   GABA (A) alpha1 Receptor (extracellular) Antibody   

GABA (A) alpha1 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GABA (A) alpha1 Receptor (extracellular) Antibody AG1279-025
    Western blot analysis of rat brain lysates:
    1. Anti-GABA(A) α1 Receptor (extracellular) antibody (#AG1279), (1:1000).
    2. Anti-GABA (A) α1 Receptor (extracellular) antibody preincubated with the control peptide antigen.
  • IHC - GABA (A) alpha1 Receptor (extracellular) Antibody AG1279-025
     Expression of GABA(A) α1 Receptor in mouse hippocampus
    Immunohistochemical staining of GABA (A) α1 Receptor in mouse hippocampus using Anti-GABA (A) α1 Receptor (extracellular) antibody (#AG1279). A. Distribution of GABA (A) α1 Receptor (red) in mouse hippocampus. B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABA (A) α1 Receptor is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
  • IHC - GABA (A) alpha1 Receptor (extracellular) Antibody AG1279-025
    Colocalization of CaV1.2 and GABA(A) α1 Receptor in rat cerebellum Immunohistochemical staining of rat cerebellum using Anti-CaV1.2 antibody (#AGP-001) and Anti-GABA(A) α1 Receptor (extracellular) antibody (#AG1279). A. CaV1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABA(A) α1 Receptor (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between CaV1.2 and GABA(A) α1 Receptor  in the granule layer.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62813
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51754 Da
Homology Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical.
Additional Information
Gene ID 29705
Other Names Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, Gabra1, Gabra-1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABA (A) ־±1 Receptor (Accession P62813). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Mouse brain lysates (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Rat brain lysates (see Woo, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse brain sections (1:500) (see Seo, S. and Leitch, B. (2014) in Product Citations). - Rat brain sections (1:250) (see Park, H.J. et al. (2013) in Product Citations).   Immunocytochemistry (IC): - Rat adrenal medullary cells (1:100) (see Matsuoka, H. et al. (2008) in Product Citations).  Live cell imaging (LCI): - Rat living hippocampal neurons (1:400) (see Pribiag, H. and Stellwagen, D. (2013) in Product Citations). - Rat living hippocampal neurons (1:500) (see Chaumont, S. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1   The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2 GABAARs are heteropentamers, in which all five subunits contribute to pore formation.  To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands. Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5


References 1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715. 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. 3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. 4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057. 5. Fuchs, K. and Celepirovic, N. (2002) J. Neurochem. 82, 1512.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 375.00
$ 475.00
$ 575.00
Cat# AG1279-025
(40 western blots)
Availability: 2-3 weeks
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions