|Calculated MW||42707 Da|
|Homology||Bovine- identical; human, canine - 13/14 amino acid residues identical.|
|Other Names||Urotensin-2 receptor, UR-2-R, G-protein coupled receptor 14, G-protein coupled sensory epithelial neuropeptide-like receptor, SENR, Urotensin II receptor, UR-II-R, Uts2r, Gpr14, Senr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RPLDTVQRSKGYRK, corresponding to amino acid residues 154-167 of rat UTIIR (Accession P49684).ֲ 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Urotensin II is a very powerful vasoconstrictive substance. The human Urotensin II receptor (UTIIR) was originally isolated as an orphan receptor (GPR14) in neural and sensory tissues.1,2 Urotensin II is expressed in various human tissues such as CNS, skeletal muscle, smooth muscle, pancreas, and heart.3-5 Binding of Urotensin II to the receptor leads to activation of phospholipase C. The resulting increase in intracellular Ca2+ may cause the contraction of vascular smooth muscle. ERK1/2 phophorylation may also be involved in mediating the biological activities of urotensin II.6 Recently, evidence has accumulated indicating that Urotensin II is also expressed in various kinds of tumor cells such as of adrenal tumors and neuroblastomas. In those cells Urotensin II was reported to stimulate cell growth.7-9 Abgent is pleased to offer a highly specific antibody directed against the 2nd cytoplasmic loop of the rat UTIIR. Anti-Urontenisn II Receptor antibody (#AG1284) can be used in Western blot anlaysis and immunohistochemical applications, and recognizes UTIIR in rat samples.
References 1. Davenport, A.P. et al. (2002) Pharmacol. Rev. 54, 219. 2. Boivin, B. et al. (2003) J. Biol.Chem. 278, 29153. 3. Maguire, J.J. et al. (2000) Br. J. Pharmacol. 131, 441. 4. Matsushita, M. et al. (2001) J. Hypertens. 19, 2185. 5. Maguire, J.J. and Davenport, A.P. (2002) Br. J. Pharmacol. 137, 57. 6. Ziltener, P. et al. (2002) J. Recept. Signal Transduct. Res. 22, 155. 7. Takahashi, K. et al. (2000) Clin. Sci. 103, 35S. 8. Takahashi, K. et al. (2001) Peptides 22, 1175. 9. Takahashi, K. et al. (2003) Peptides 24, 301.
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