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ET-B Antibody

Affinity purified polyclonal antibody

  • WB - ET-B Antibody AG1285-025
    Western blot analysis of rat brain membranes: 1.  Anti-Endothelin Receptor B antibody (#AG1285), (1:200).
    2.  Anti-Endothelin Receptor B antibody, preincubated with the control peptide antigen.
  • IHC - ET-B Antibody AG1285-025
    Expression of ET-B in rat lung Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor B antibody (#AG1285) (1:100). ET-B is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Note that vascular smooth muscle is not stained (blue arrows). Hematoxilin is used as the counterstain.
  • IHC - ET-B Antibody AG1285-025
    Expression of ET-B in rat thalamus Immunohistochemical staining of rat dorsal peri-ventricular thalamus using Anti-Endothelin Receptor B antibody (#AG1285).  Cresyl violet is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21451
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 49455 Da
Homology All known vertebrate sequences - identical.
Additional Information
Gene ID 50672
Other Names Endothelin B receptor, ET-B, ET-BR, Endothelin receptor non-selective type, Ednrb
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B (Accession P21451). 3rd intracellularֲ loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Immunohistochemistry (IH): - Mouse retina (see Bramall, A.N. et al. (2013) in Product Citations). - Mouse retina (1:100) (see Rattner, A. and Nathans, J. (2005) in Product Citations).   Immunocytochemistry (IC): - Human endocardial endothelial cells (EECs) (1:100) (see Jacques, D. et al.(2005) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein coupled receptors, ETAR and ETBR. Both ETAR and ETBR are present in the heart and in human myocardium at similar levels.1,2 The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3  Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities. Currently there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3 Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ETAR and ETBR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane.2 Hypermethylation of the ETBR correlated with transcriptional down-regulation and reduced expression of ETB receptor was observed in several prostate, bladder and colon cancer cell lines.3


References 1. Davenport, A.P. (2002) Pharmacol. Rev. 54, 219. 2. Boivin, B. et al. (2003) J. Biol.Chem. 278, 29153. 3. Grant, K. et al. (2003) Br. J. Cancer. 88, 163.

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Cat# AG1285-025
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