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ET-A Antibody

Affinity purified polyclonal antibody

  • WB - ET-A Antibody AG1286-025
    Western blot analysis of rat brain membranes: 1. Anti-Endothelin Receptor A antibody (#AG1286), (1:200).
    2. Anti-Endothelin Receptor A antibody, preincubated with the control peptide antigen.
  • IHC - ET-A Antibody AG1286-025
    Expression of ET-A in rat lung Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor A antibody (#AG1286), (1:100). ET-A is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Hematoxilin is used as the counterstain.
  • IHC - ET-A Antibody AG1286-025
    Expression of ET-A in rat cerebellum  Immunohistochemical staining of rat cerebellum using Anti-Endothelin Receptor A antibody (#AG1286) (A), and Anti-ET-A antibody preincubated with the control peptide antigen (B). Cresyl violet is used as the counterstain. 
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P26684
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 48245 Da
Homology Human, swine, bovine - 13/14 amino acid residues identical; chicken - 12/14 amino acid residues identical.
Additional Information
Gene ID 24326
Other Names Endothelin-1 receptor, Endothelin A receptor, ET-A, ET-AR, Ednra
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A (Accession P26684). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Immunohistochemistry (IH): - Mouse retina (see Bramall, A.N. et al. (2013) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.9 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2 The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities. Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3 Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi’s sarcoma, ovarian and breast carcinomas.4-9 In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer15 suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.


References 1. Davenport, A.P. (2002) Pharmacol. Rev. 54, 219. 2. Boivin, B. et al. (2003) J. Biol.Chem. 278, 29153. 3. Grant, K. et al. (2003) Br. J. Cancer. 88, 163. 4. Asham, E. et al. (2001) Br. J. Cancer. 85, 1759. 5. Kopetz, E.S. et al. (2002) Invest. New Drugs. 20, 173. 6. Rosano, L. et al. (2003) Am. J. Pathol. 163, 753. 7. Rosano, L. et al. (2003) Cancer Res. 63, 2447. 8. Wulfing, P. et al. (2003) Clin. Cancer Res. 9, 4125. 9. Nelson, J.B. (2003) J. Urol. 170, S65.

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Cat# AG1286-025
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