|Application ||WB, IHC|
|Calculated MW||52831 Da|
|Homology||Mouse - 12/13 amino acid residues identical.|
|Other Names||D(1B) dopamine receptor, D(5) dopamine receptor, Dopamine D5 receptor, Drd5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat D5 dopamine receptor (Accession P25115). 2nd extracellular loop.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||Add 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The D5 Dopamine Receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.1 All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1 The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract. The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knock out mice have shown no overt alterations in locomotor or cognitive functions. However, knock out mice do develop severe hypertension suggesting a role of D5 receptor in the modulation of neuronal pathways regulating blood pressure responses.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat D5 dopamine receptor. Anti-D5 Dopamine Receptor (extracellular) antibody (#AG1287) can be used in wstern blot analysis and immunohistochemical applications, and recognizes D5 dopamine receptor from rat and mouse samples.
1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
2. Hollon, T.R. et al. (2002) J. Neurosci. 22,1080.
3. Holmes, A. et al. (2004) Neuropharmacol. 47,111.
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