Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   D3 Dopamine Receptor (extracellular) Antibody   

D3 Dopamine Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - D3 Dopamine Receptor (extracellular) Antibody AG1289-025
    Western blot analysis of rat whole brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: 1, 2. Anti-D3 Dopamine Receptor (extracellular) antibody (#AG1289), (1:200). 3, 4. Anti-D3 Dopamine Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - D3 Dopamine Receptor (extracellular) Antibody AG1289-025
    Western blot analysis of mouse brain membranes: 1. Anti-D3 Dopamine Receptor (extracellular) antibody (#AG1289), (1:200). 2. Anti-D3 Dopamine Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - D3 Dopamine Receptor (extracellular) Antibody AG1289-025
    Expression of D3 Dopamine Receptor in rat striatum Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3 Dopamine Receptor (extracellular) antibody (#AG1289), (1:100). A. D3 Dopamine receptor appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that D3 Dopamine Receptor localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19020
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 49516 Da
Homology Mouse - identical; human - 12/15 amino acid residues identical.
Additional Information
Gene ID 29238
Other Names D(3) dopamine receptor, Dopamine D3 receptor, Drd3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat D3 Dopamine receptor (Accession P19020). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution Add 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.1 All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1 The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle. In the periphery, it is expressed in the kidney particularly in proximal tubules.1 The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat D3 dopamine receptor. Anti-D3 Dopamine Receptor (extracellular) antibody (#AG1289) can be used in western blot analysis and immunohistochemical applications, and recognizes D3 dopamine receptor from rat and mouse samples


1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
2. Holmes, A. et al. (2004) Neuropharmacol. 47, 1117.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 375.00
$ 475.00
$ 575.00
Cat# AG1289-025
(40 western blots)
Availability: 2-3 weeks
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions