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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Choline Transporter (CHT) (extracellular) Antibody   

Choline Transporter (CHT) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Choline Transporter (CHT) (extracellular) Antibody AG1292-050
    Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates: 1-3. Anti-Choline Transporter  (CHT)  (extracellular) antibody (#AG1292), (1:200).
    4-6. Anti-Choline Transporter  (CHT)  (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Choline Transporter (CHT) (extracellular) Antibody AG1292-050
    Expression of Choline Transporter in rat striatum Immunohistochemical staining of rat frozen brain sections using Anti-Choline Transporter (CHT) (extracellular) antibody (#AG1292) (1:200). A. Choline Transporter expression (green) is detected in the matrix (M), but not in the patches (P). B. DAPI (blue) staining provides general cellular staining. C. merge of A and B shows that Choline Transporter appears in a subset of striatal neurons (arrows provide examples).
  • ICC - Choline Transporter (CHT) (extracellular) Antibody AG1292-050
    Expression of Choline Transporter in rat PC12 cells Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Choline Transporter (CHT) (extracellular) antibody (#AG1292), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BGY9
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 63365 Da
Homology Rat, human - 12/13 amino acid residues identical.
Additional Information
Gene ID 63993
Other Names High affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7, Slc5a7, Cht1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline Transporter (Accession Q8BGY9). 4th extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released in the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine released in the synaptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2. CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e., increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2. Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons5. Abgent is pleased to offer a highly specific antibody directed against an epitope of mouse CHT. Anti-Choline Transporter (CHT) (extracellular) antibody (#AG1292) can be used in western blot analysis and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.


References 1. Ribeiro, F.M. et al. (2006) J. neurochem. 97, 1. 2. Okuda, T. and Haga, T. (2003) Neurochem. Res. 28, 483. 3. Apparsundaram, S. et al. (2000) Biochem. Biophys. Res. Commun. 276, 862. 4. Ferguson, S.M. and Blakely, R.S. (2004) Mol. Intervent. 4, 22. 5. Simon, J.R. and Kuhar, M.G. (1975) Nature 255, 162. 6. Misawa, H. et al. (2001) Neuroscience 105, 87.

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$ 475.00
$ 575.00
Cat# AG1292-050
(40 western blots)
Availability: 2-3 weeks
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