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Cholecystokinin B Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Cholecystokinin B Receptor (extracellular) Antibody AG1294-050
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 1, 2. Anti-Cholecystokinin B Receptor (extracellular) antibody (#AG1294), (1:200). 3, 4. Anti-Cholecystokinin B Receptor (extracellular), preincubated with the control peptide antigen.
  • IHC - Cholecystokinin B Receptor (extracellular) Antibody AG1294-050
    Expression of Cholecystokinin B Receptor in rat stomach Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-Cholecystokinin B Receptor (extracellular) antibody (#AG1294), (1:100). Cholecystokinin B Receptor (brown) is expressed in both parietal cells (black arrows) and in chief cells (red arrows) of the gastric mucosa. Hematoxilin is used as the counterstain.
  • FC - Cholecystokinin B Receptor (extracellular) Antibody AG1294-050
    Indirect flow cytometry analysis in live intact Jurkat (human T cell leukemia) cell lines:
    Black: Unstained cells + goat-anti-rabbit-Phycoerythrin (PE).
    Green: Cells + Anti-Cholecystokinin B Receptor (extracellular) antibody (#AG1294), (1:20) + goat-anti-rabbit-PE.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56481
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 49172 Da
Homology Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
Additional Information
Gene ID 12426
Other Names Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, Cckbr
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Cholecystokinin B Receptor (Accession P56481). Extracellular, N-terminus.
Dilution WB~~1:200
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution Add 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Cholecystokinin B Receptor (CCKBR) also known as the Gastrin Receptor and CCKR2, belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily, and is one of the two receptors that mediates the effects of the cholecystokinin (CCK) and gastrin peptides1,2. CCK and gastrin are related linear peptides that occur in different forms but share the last five C-terminal amino acid residues. CCK regulates several nutritional-related activities such as stimulation of pancreatic exocrine secretion or the regulation of intestinal transit, while gastrin's main function is the stimulation of gastric acid secretion1,2. Both CCKBR and the other CCK receptor (CCKAR or CCK1R) are coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), and intracellular Ca2+ mobilization. CCKBR was originally identified in the brain (and hence its name, type B for Brain) where it is widely distributed, notably in the cerebral cortex and striatum. In the periphery, CCKBR is most notably expressed in acid secreting cells in the mucosa of the stomach. The tissue distribution of CCKBR corresponds to the proposed roles of the receptor which include anxiety, pain perception, gastric acid secretion, and growth and differentiation of the gastric mucosa1,2. Abgent is pleased to offer a highly specific antibody directed against an epitope in the extracellular N-terminus of the mouse CCKBR. Anti-Cholecystokinin B Receptor (extracellular) antibody (#AG1294) can be used in Western blot, indirect flow cytometry and immunohistochemical applications, and recognizes CCKBR from mouse, rat and human samples.


1. Noble, F. et al. (1999) Pharmacol. Rev. 51, 745.
2. Dufresne, M. et al. (2006) Physiol Rev. 86, 805.

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$ 475.00
$ 575.00
Cat# AG1294-050
(40 western blots)
Availability: 2-3 weeks
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