|Application ||WB, IHC, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49172 Da|
|Homology||Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.|
|Other Names||Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, Cckbr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Cholecystokinin B Receptor (Accession P56481). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||Add 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cholecystokinin B Receptor (CCKBR) also known as the Gastrin Receptor and CCKR2, belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily, and is one of the two receptors that mediates the effects of the cholecystokinin (CCK) and gastrin peptides1,2. CCK and gastrin are related linear peptides that occur in different forms but share the last five C-terminal amino acid residues. CCK regulates several nutritional-related activities such as stimulation of pancreatic exocrine secretion or the regulation of intestinal transit, while gastrin's main function is the stimulation of gastric acid secretion1,2. Both CCKBR and the other CCK receptor (CCKAR or CCK1R) are coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), and intracellular Ca2+ mobilization. CCKBR was originally identified in the brain (and hence its name, type B for Brain) where it is widely distributed, notably in the cerebral cortex and striatum. In the periphery, CCKBR is most notably expressed in acid secreting cells in the mucosa of the stomach. The tissue distribution of CCKBR corresponds to the proposed roles of the receptor which include anxiety, pain perception, gastric acid secretion, and growth and differentiation of the gastric mucosa1,2. Abgent is pleased to offer a highly specific antibody directed against an epitope in the extracellular N-terminus of the mouse CCKBR. Anti-Cholecystokinin B Receptor (extracellular) antibody (#AG1294) can be used in Western blot, indirect flow cytometry and immunohistochemical applications, and recognizes CCKBR from mouse, rat and human samples.
1. Noble, F. et al. (1999) Pharmacol. Rev. 51, 745.
2. Dufresne, M. et al. (2006) Physiol Rev. 86, 805.
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