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CCR7 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • FC - CCR7 (extracellular) Antibody AG1295-050
    Flow cytometric analysis of human peripheral blood lymphocytes cells using Anti-CCR7 (extracellular) (green, Cat#AG1295) compared to an isotype control of rabbit IgG (blue). AG1295 was diluted at 1:80 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - CCR7 (extracellular) Antibody AG1295-050
    Western blot analysis of Jurkat (lanes 1 and 4), MOLT-4 (lanes 2 and 5) and J774A.1 (lanes 3 and 6) cell lysates: 1-3. Anti-CCR7 (extracellular) antibody (#AG1295), (1:200). 4-6. Anti-CCR7 (extracellular) antibody, preincubated with the control peptide antigen.
  • FC - CCR7 (extracellular) Antibody AG1295-050
    Indirect flow cytometry analysis of Jurkat living cells:
    Black: Unstained cells.
    Green: Cells + Anti-CCR7 (extracellular) antibody, (#AG1295) (5-10 µg/1x106 cells) followed by goat anti-rabbit-dylight 488.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P32248
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 42874 Da
Homology Rat, mouse - 14/15 amino acid residues identical.
Additional Information
Gene ID 1236
Other Names C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, CCR7, CMKBR7, EBI1, EVI1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7 (Accession P32248). Extracellular, N-terminus.
Dilution FC~~1:80
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2. All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3. Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8. In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection. CCR7 receptor is responsible for directing dendritic cell migration to the lymph nodes where they are important for initiating the immune response. This receptor has two ligands, CCL19 and CCL21 which are expressed by stromal cells in the T cell-rich lymph node area9-11. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CCR7. Anti-CCR7 (extracellular) antibody (#AG1295) can be used in western blot analysis and indirect flow cytometry applications and has been designed to recognize CCR7 from mouse, rat and human samples.


1. Jin, T. et al. (2008) Cytokine 44, 1.
2. Murphy, P.M. (2002) Pharmacol. Rev. 54, 227.
3. Viola, A. and Luster, A.D. (2007) Annu. Rev. Pharmacol. Toxicol. 48, 171.
4. Cardona, A.E. et al. (2008) J. Leukoc. Biol. 84, 587.
5. Lodowski, D.T. and Palczewski, K. (2009) Curr. Opin. HIV AIDS 4, 88.
6. Tran, P.B. and Miller, R.J. (2003) Nat. Rev. Neurosci. 4, 444.
7. Moser, B. and Loetscher, P. (2001) Nat. Immunol. 2, 123.
8. Pals, S.T. et al. (2007) Blood 110, 3102.
9. Sanchez-Sanchez, N. et al. (2006) J. Immunol. 176, 5153.
10. Gunn, M.D. (2003) Semin. Immunol. 15, 271.
11. Randolph, G.J. et al. (2005) Nat. Rev. Immunol. 5, 617.

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$ 367.00
$ 475.00
Cat# AG1295-050
Availability: 2-3 weeks
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