|Application ||FC, WB|
|Calculated MW||40759 Da|
|Other Names||C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8R B, IL-8 receptor type 2, CD182, CXCR2, IL8RB|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDFNMESDSFEDFWKGED, corresponding to amino acid residues 2-19 of human CXCR2 (Accession P25025). Extracellular, N-terminus.Will only recognize CXCR2 from human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.55 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2. All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3. Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8. In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection. The CXCR2 receptor could mediate chemotaxis and degranulation of neutrophils along with CXCR1, via interleukin-8 (IL-8) a proinflammatory chemokine with the CXC motif9. In addition, in a model of arterial injury, CXCR2 can improve endothelial recovery, and also seems to be important in recruiting endothelial progenitor cells to injured vessels10. CXCR2 is activated by a plethora of ligands: GROa, b, g, neutrophil-activating peptide, granulocyte chemotactic protein-2 and keratinocyte derived chemokine (CXCL1). CXCR2 is expressed in different brain regions11, in the lung and spleen12 and other regions. Abgent is pleased to offer a highly specific antibody directed against an epitope located at N-terminus extracellular domain of human CXCR2. Anti-CXCR2 (extracellular) antibody (#AG1297) can be used in western blot and indirect flow cytometry applications, and has been designed to recognize CXCR2 from human samples.
1. Jin, T. et al. (2008) Cytokine 44, 1.
2. Murphy, P.M. (2002) Pharmacol. Rev. 54, 227.
3. Viola, A. and Luster, A.D. (2007) Annu. Rev. Pharmacol. Toxicol. 48, 171.
4. Cardona, A.E. et al. (2008) J. Leukoc. Biol. 84, 587.
5. Lodowski, D.T. and Palczewski, K. (2009) Curr. Opin. HIV AIDS 4, 88.
6. Tran, P.B. and Miller, R.J. (2003) Nat. Rev. Neurosci. 4, 444.
7. Moser, B. and Loetscher, P. (2001) Nat. Immunol. 2, 123.
8. Pals, S.T. et al. (2007) Blood 110, 3102.
9. Waugh, D.J.J. and Wilson, C. (2008) Clin. Cancer Res. 14, 6735.
10. Page, E.L. et al. (2002) J. Biol. Chem. 277, 48403.
11. Horuk, R. et al. (1997) J. Immunol. 158, 2882.
12. Dunstan, C.A. et al. (1996) J. Biol. Chem. 271, 32770.
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