|Application ||FC, WB|
|Calculated MW||39791 Da|
|Homology||Specific to human CXCR1.|
|Other Names||C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8R A, IL-8 receptor type 1, CD181, CXCR1, CMKAR1, IL8RA|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SNITDPQMW DFDDLN, corresponding to aminoacid residues 2-16 of human CXCR1 (Accession P25024). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Chemokine receptors belong to the seven transmembrane domain G-Protein Coupled Receptors and are differentially expressed in diverse cell types. In leukocytes, chemokines coordinate development, differentiation, anatomic distribution, trafficking, and effector functions and thereby regulate innate and adaptive immune responses. To date, 18 chemokine receptors are known and designated as CXCR1-5, CCR1-11, XCR1, and CX3CR1 based on their specific chemokine preferences. The CXC chemokine receptors specifically bind and respond to cytokines of the CXC chemokine family. CXCR1 and CXCR2 were the first chemokine receptor subtypes to be defined. They are the only known mammalian receptors for ELR+ CXC chemokines [CXC chemokines which contain the tripeptide motif glutamic acid-leucine-arginine (ELR) N-terminal to the first cysteine]. CXCR1 and CXCR2 both bind IL-8, with similar high affinity; they do not bind other types of chemokines. CXCR1 and CXCR2 are the major chemokine receptors expressed on neutrophils. They appear to operate mainly in acute inflammation and innate immunity. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human CXCR1. Anti-CXCR1 (extracellular) antibody (#AG1298) can be used for western blot analyses and indirect flow cytometry applications. It was specifically designed to recognize human CXCR1.
1. Murphy, P.M. et al. (2000) Pharmacol. Rev. 52, 145.
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