|Calculated MW||39681 Da|
|Homology||Canis, bovine- 10/14 amino acid residues identical.|
|Other Names||Cannabinoid receptor 2, CB-2, CB2, hCB2, CX5, CNR2, CB2A, CB2B|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human Cannabinoid Receptor 2 (Accession P34972). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cannabinoids have been used as pain relievers in eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: the Cannabinoid Receptor 1 (CB1R) and the Cannabinoid Receptor 2 (CB2R).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3 The CB2R is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2R expression in brain is still much less characterized than that of CB1R. Recently, it was demonstrated that CB2R is expressed in brain and might have role in controlling fundamental processes such as proliferation and survival of neural cells.5,6 Overexpression of CB2R was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2R expression and tumor malignancy was demonstrated.8 Activation of CB2R, in vivo, by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma it was shown that activation of the CB2R by JWH-133 resulted in internalization of only the CB2R, and not CB1R leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma. Alomone is pleased to offer a new, extracellular antibody directed against the human CB2R. Anti-Cannabinoid Receptor 2 (extracellular) antibody (#AG1299) can be used in western blot and immunocytochemistry applications, and recognizes CB2R in human samples.
References 1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth. 3, 175. 2. Howlett, A.C. (2002) Prostaglandins & other Lipid Mediators 68, 619. 3. Guo, J. and Ikeda, S.R. (2004) Mol. Pharmacol. 65, 665. 4. Wotherspoon, G. et al. (2005) Neuroscience 135, 235. 5. Gong, J.P. et al. (2006) Brain Research 1071, 10. 6. Fernandez-Ruiz, J. et al. (2007) Trends Pharmacol. Sci. 28, 39. 7. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635. 8. Sánchez, C. et al. (2001) Cancer Res. 61, 5784. 9. Jordà, M.A. et al. (2004) Blood 104, 526.
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