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Cannabinoid Receptor 1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Cannabinoid Receptor 1 (extracellular) Antibody AG1300-025
    Western blot analysis of rat brain membrane proteins: 1. Anti-Cannabinoid Receptor 1 (extracellular) antibody (#AG1300), (1:200).
    2. Anti-Cannabinoid Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Cannabinoid Receptor 1 (extracellular) Antibody AG1300-025
    Expression of CB1R in mouse hippocampus Immunohistochemical staining of mouse hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) antibody (#AG1300), (1:100). A. CB1R is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1R in GABAergic cells.
  • IHC - Cannabinoid Receptor 1 (extracellular) Antibody AG1300-025
    Expression of CB1R in rat hippocampus Immunohistochemical staining of rat hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) antibody (#AG1300) (1:100). A. CB1R is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1R in GABAergic cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20272
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 52845 Da
Homology Human - identical; mouse - 15/16 amino acid residues identical.
Additional Information
Gene ID 25248
Other Names Cannabinoid receptor 1, CB-R, CB1, Brain-type cannabinoid receptor, Cnr1, Skr6
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.7 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Cannabinoids have been used in eastern medicine for many years as pain relievers.1 Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 Receptor or CB1R) and cannabinoid receptor 2 (CB2 Receptor or CB2R).2 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.2 Both receptors belong to the G-protein-coupled receptor (GPCR) superfamily. CB1R was shown to modulate several Ca2+ and K+ ion channels.2,3 CB1R is primarily expressed in the central nervous system. However, expression of CB1R is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.3,4 CB1R is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.5,6 Growth inhibition of tumor cells was demonstrated following mixed CB1R/CB2R agonist treatment in both prostate and non-melanoma skin cancers.5,6 Through their interaction with CB1R, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1R an attractive therapeutic target for the treatment of obesity and eating disorders.7 Alomone is pleased to offer a new extracellular antibody directed against the rat CB1 receptor. Anti-Cannabinoid Receptor 1 (extracellular) antibody (#AG1300) can be used in western blot and immunohistochemistry applications, and recognizes CB1R in mouse and rat samples.


References 1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth. 3, 175. 2. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68–69, 619. 3. Howlett, A.C. et al. (2002) Pharmacol. Rev. 54, 161. 4. Rueda, D. et al. (2000) Mol. Pharmacol. 58, 814. 5. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635. 6. Casanova, M.L. et al. (2003) J. Clin. Invest. 111, 43. 7. Cota, D. et al. (2003) Int. J. Obes. Relat. Metab. Disord. 27, 289.

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Cat# AG1300-025
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