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SERCA2 Antibody

Affinity purified polyclonal antibody

  • WB - SERCA2 Antibody AG1301-050
    Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8): 1- 4. Anti-SERCA2 antibody (#AG1301), (1:200).
    5- 8. Anti-SERCA2  antibody, preincubated with the control peptide antigen.
  • ICC - SERCA2 Antibody AG1301-050
    Expression of SERCA1 in mouse C2C12 cells Immunocytochemical staining of fixed and permeabilized mouse myoblast C2C12 cells. A. Cells were stained using Anti-SERCA2 antibody (#AG1301), (1:300) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merged image of SERCA2 labeling with a live image of the cells.  Cell nuclei were visualized using Hoechst 33342 (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11507
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 114768 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 29693
Other Names Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Atp2a2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CTPNKPSRTSMSK, corresponding to amino acid residues 498- 510 of rat SERCA2 (Accession P11507). 2nd cytoplasmic loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1. In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.   SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.


References 1. Brini, M. and Carafoli, E. (2009) Physiol. Rev. 89, 1341. 2. Vandecaetsbeek, I. et al. (2011) Cold Spring Harb. Perspect. Biol. 3, a004184. 3. Clausen, J.D. et al. (2012) J. Biol. Chem. 287, 39460. 4. Shi, B.J. et al. (2012) Int. J. Dermatol. 51, 1074.

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$ 475.00
$ 575.00
Cat# AG1301-050
(40 western blots)
Availability: 2-3 weeks
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