|Calculated MW||136811 Da|
|Homology||Mouse - identical; human - 13/15 amino acid residues identical.|
|Other Names||Plasma membrane calcium-transporting ATPase 2, PMCA2, Plasma membrane calcium ATPase isoform 2, Plasma membrane calcium pump isoform 2, Atp2b2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2 (Accession P11506). 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The plasma membrane Ca2+ ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1. The PMCA and the Na+ calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2. Since it transports Ca2+ into the extracellular space, the PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3. The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4. The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, so it is suited for maintaining Ca2+ at its normally very low levels. The NCX is better suited for removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.
References 1. Jensen, T.P. et al. (2004) Brain Res. Dev. Brain Res. 152, 129. 2. Strehler, E.E. and Zacharias, D.A. (2001) Physiol. Rev. 81, 21. 3. Talarico, E.F. et al. (2005) Mol. Vis. 11, 169. 4. Jensen, T.P. et al. (2007) J. Physiol. 579, 85. 5. Burette, A. and Weinberg, R.J. (2007) J. Comp. Neurol. 500, 1127.
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