|Calculated MW||28299 Da|
|Homology||Mouse - identical; human - 9/14 amino acid residues identical.|
|Other Names||Chloride intracellular channel protein 5, Clic5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161- 174 of rat CLIC5 (Accession Q9EPT8). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Chloride (Cl-) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl- channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity; they may reside in either the plasma membrane or the membrane of intracellular compartments or both1. They are detected in the kidney, placenta, intestines, brain and the inner-ear1-4. The vertebrate Cl- intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6); each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel1,5. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl-5. CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene5, has first been isolated from human placenta6, and was shown, together with CLIC4 (with which it shares 76% homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton4. CLIC5 shares about 40% homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear2,4. Mice mutated in the Clic5 gene lack coordination and gradually become deaf2, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat CLIC5. Anti-CLIC5 antibody (#AG1304) can be used in western blot analysis. It has been designed to recognize CLIC5 from mouse, rat and human samples.
References 1. Jentsch, T.J. et al. (2002) Physiol. Rev. 82, 503. 2. Gagnon, L.H. et al. (2006) J. Neurosci. 26, 10188. 3. Wegner, B. et al. (2010) Am. J. Physiol. 298, F1492. 4. Berryman, M. and Bretscher, A. (2001) Mol. Biol. Cell 11, 1509. 5. Littler, D.R. et al. (2010) FEBS Letters 584, 2093. 6. Debska, G. et al. (2001) Acta Biochim. Polonica 48, 137. 7. Bradford, E.M. et al. (2010) Am. J. Physiol. 298, R1531.
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