|Reactivity||Human, Mouse, Rat|
|Calculated MW||28772 Da|
|Homology||Rat, mouse- identical.|
|Other Names||Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8 - 20 of human CLIC4 (Accession Q9Y696).ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3. The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain1. When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6. CLIC4 distribution is rather ubiquitous but shows higher expression level in skin, kidney, brain and testes7. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CLIC4. Anti-CLIC4 antibody (#AG1305) can be used in western blot and immunocytochemistry applications and has been designed to recognize CLIC4 from mouse, rat and human samples.
References 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102. 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1. 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356. 4. Shukla, A. et al. (2009) Nat. Cell Biol. 11, 777. 5. Suh, K.S. et al. (2007) J. Cell Sci. 120, 2631. 6. Suh, K.S. et al. (2004) J. Biol. Chem. 279, 4632. 7. Shukla, A. and Yuspa, S.H. (2010) Nucleus 1, 144.
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