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CLIC4 Antibody

Affinity purified polyclonal antibody

  • WB - CLIC4 Antibody AG1305-050
    Western blot analysis of human pancreatic carcinoma PANC-1 (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat kidney (lanes 3 and 6) lysates:
    1- 3. Anti-CLIC4 antibody (#AG1305), (1:200).
    4- 6. Anti-CLIC4 antibody, preincubated with the control peptide antigen.
  • ICC - CLIC4 Antibody AG1305-050
    Expression of CLIC4 in human pancreatic carcinoma PANC-1 cells Immunocytochemical staining of fixed and permeabilized human pancreatic carcinoma PANC-1 cells. A. Cells were stained with Anti-CLIC4 antibody (#AG1305), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live image of the cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y696
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28772 Da
Homology Rat, mouse- identical.
Additional Information
Gene ID 25932
Other Names Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8 - 20 of human CLIC4 (Accession Q9Y696).ֲ Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3. The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain1. When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6. CLIC4 distribution is rather ubiquitous but shows higher expression level in skin, kidney, brain and testes7. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CLIC4. Anti-CLIC4 antibody (#AG1305) can be used in western blot and immunocytochemistry applications and has been designed to recognize CLIC4 from mouse, rat and human samples.


References 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102. 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1. 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356. 4. Shukla, A. et al. (2009) Nat. Cell Biol. 11, 777. 5. Suh, K.S. et al. (2007) J. Cell Sci. 120, 2631. 6. Suh, K.S. et al. (2004) J. Biol. Chem. 279, 4632. 7. Shukla, A. and Yuspa, S.H. (2010) Nucleus 1, 144.

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$ 367.00
$ 475.00
Cat# AG1305-050
Availability: 2-3 weeks
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