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CLIC1 Antibody

Affinity purified polyclonal antibody

  • WB - CLIC1 Antibody AG1306-050
    Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates: 1- 4. Anti-CLIC1 antibody (#AG1306), (1:200).
    5- 8. Anti-CLIC1 antibody, preincubated with the control peptide antigen.
  • ICC - CLIC1 Antibody AG1306-050
    Expression of CLIC1 human colorectal adenocarcinoma HT-29 cells Immunocytochemical staining of fixed and permeabilized human colorectal adenocarcinoma HT-29 cells. A. Cells were stained with Anti-CLIC1 antibody (#AG1306), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00299
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26923 Da
Homology Rat, mouse- identical.
Additional Information
Gene ID 1192
Other Names Chloride intracellular channel protein 1, Chloride channel ABP, Nuclear chloride ion channel 27, NCC27, Regulatory nuclear chloride ion channel protein, hRNCC, CLIC1, G6, NCC27
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3. The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain1. When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic1. CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CLIC1. Anti-CLIC1 antibody (#AG1306) can be used in western blot and immunocytochemistry applications and has been designed to recognize CLIC1from mouse, rat and human samples.


References 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102. 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1. 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356. 4. Ulmasov, B. et al. (2007) BMC Cell. Biol. 8, 1. 5. Jung, J.J. and Kitajewski, J. (2010) J. Angiogenes. Res. 2, 23.

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$ 367.00
$ 475.00
Cat# AG1306-050
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