|Calculated MW||26923 Da|
|Homology||Rat, mouse- identical.|
|Other Names||Chloride intracellular channel protein 1, Chloride channel ABP, Nuclear chloride ion channel 27, NCC27, Regulatory nuclear chloride ion channel protein, hRNCC, CLIC1, G6, NCC27|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3. The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain1. When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic1. CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CLIC1. Anti-CLIC1 antibody (#AG1306) can be used in western blot and immunocytochemistry applications and has been designed to recognize CLIC1from mouse, rat and human samples.
References 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102. 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1. 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356. 4. Ulmasov, B. et al. (2007) BMC Cell. Biol. 8, 1. 5. Jung, J.J. and Kitajewski, J. (2010) J. Angiogenes. Res. 2, 23.
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