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CFTR Antibody

Affinity purified polyclonal antibody

  • WB - CFTR Antibody AG1308-025
    Western blot analysis of rat lung membranes:
    1. Anti-CFTR antibody (#AG1308), (1:200).
    2. Anti-CFTR antibody, preincubated with the control peptide antigen.
  • IHC - CFTR Antibody AG1308-025
    Expression of CFTR in rat lungs Immunohistochemical staining of rat lungs sections using Anti-CFTR antibody (# AG1308) (left panel). Strong staining of bronchial epithelial cells (red) and lighter staining of alveolar cells (red-brown) is apparent. There is also positive staining of macrophages while smooth muscle and endothelium are negative. Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13569
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 168142 Da
Homology Mouse, rat, pig - 13/14 amino acid residues identical.
Additional Information
Gene ID 1080
Other Names Cystic fibrosis transmembrane conductance regulator, CFTR, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel, CFTR, ABCC7
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 of human CFTR (Accession #P13569).ֲ ֲ Cytoplasmic, C-terminal part.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The cystic fibrosis transmembrane conductance regulator (CFTR) is the most dominant Cl- channel in several epithelial tissues, especially in lung and colon. Remarkably, CFTR is a member of the ATP-binding cassette (ABC) transporter superfamily that uses ATP hydrolyzation as the driving force for the translocation of a wide variety of substrates including sugars, amino acids, proteins and hydrophobic compounds, across cellular membranes. The CFTR is unique among ABC transporters in that it is a cAMP-regulated Cl- channel. It shares the superfamily topology of 12 transmembrane domains with two nucleotide-binding domains (NBDs) and a regulatory (R) domain in the large third intracytoplasmic loop that is phosphorylated in multiple sites by PKA. Mutations in the CFTR gene cause channel dysfunction in several ways, ranging from complete loss of surface expression to diminished Cl- secretion.  Defects in the CFTR gene cause cystic fibrosis (CF), the most common genetic disease among Caucasians, as well as a form of male sterility.   Regulation of the CFTR channel is accomplished through the activation of surface receptors that couple to adenyl cyclase, raise cAMP cellular levels and thus activate PKA. This has been demonstrated for the adenosine and ß2 adrenergic receptor and the vasopressin hormone among others.   Besides enhanced Cl- conductance, activation of CFTR also leads to the regulation of other ion channels. The best-studied case is its interaction with the epithelial Na+ channels (ENaC), although it can probably regulate other ion channels as well (Kir1.1 for example). The mechanism by which CFTR regulates other ion channels is not clear, but it may involve protein-protein interactions via molecules that interact with its C-terminal PDZ binding motif, such as the NHERF adaptor protein.   Abgent is pleased to offer a newly developed Anti-CFTR antibody (#AG1308) raised against a highly specific epitope located at the C-terminus domain of the human channel. The antibody has been tested in western and immunohistochemistry applications and will also detect CFTR from rat and mouse samples.


References 1. Jentsch, T.J. et al. (2002) Physiol. Rev. 82, 503. 2. Pilewski J.M. et al. (1999) Physiol Rev 79, S215–S255. 3. Greger, R. et al. (2001) Pflugers Arch. 443(Suppl. 1), S3-S7.

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$ 247.00
$ 367.00
$ 475.00
Cat# AG1308-025
Availability: 2-3 weeks
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