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CLC-1 Antibody

Affinity purified polyclonal antibody

  • WB - CLC-1 Antibody AG1309-050
    Western blot analysis of rat skeletal muscle membranes:

    1. Anti-CLC-1 antibody (#AG1309), (1:200).
    Anti-CLC-1 antibody, preincubated with the control peptide antigen.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35524
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 110073 Da
Homology Mouse - identical; human, goat - 13/16 amino acidresidues identical.
Additional Information
Gene ID 25688
Other Names Chloride channel protein 1, ClC-1, Chloride channel protein, skeletal muscle, Clcn1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CVHRLGRVLRRKLGED, corresponding to amino acid residues 102-117 of rat CLC-1. (Accession # P35524). Cytoplasmic, N-terminal part.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.3 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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CLC-1 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7. CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2   The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2   The ClC-1 channel is almost exclusively expressed in skeletal muscle where it has a dominant role in maintaining the membrane potential at rest and is important for repolarization of the skeletal muscle cells. Mutations in the ClC-1 gene result in myotonia congenita, a condition in which an increase in the excitability of skeletal muscle leads to repetitive action potentials, stiffness, and delayed relaxation. The disease can be either autosomal dominant (Thomsen's disease), or recessive (Becker's myotonia).3   Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular N-terminal domain of rat CLC-1. Anti-CLC-1 antibody (#AG1309) can be used in western blot analysis, and recognizes the CLC-1 channel from rat samples.


References 1. Jentsch, T.J. et al. (2002) Physiol. Rev. 82, 503. 2. Babini, E. and Pusch, M. (2004) Physiology 19, 293. 3. Koch, M.C. et al. (1992) Science  257, 797.

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$ 475.00
$ 575.00
Cat# AG1309-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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