|Calculated MW||83068 Da|
|Homology||Mouse- identical; human - 14/15 amino acid residues identical.|
|Other Names||H(+)/Cl(-) exchange transporter 5, Chloride channel protein 5, ClC-5, Chloride transporter ClC-5, Clcn5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLC5ֲ (Accession number P51796 ). Extracellular.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
CLC5 is a member of the voltage-dependent Cl- channel family that includes nine known members. Based on sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits. Mutations in the CLC5 gene cause Dent's disease a X-linked renal disorder. CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes vesicles, where it is believed to contribute to their acidification. The channel is mainly expressed in the kidney, although it has been found also in small intestine, brain and liver.
References 1. Jentsch, T.J.et al. (2002) Physiol. Rev. 82, 503. 2. Piwon, N. et al. (2000) Nature 408, 369.
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