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CatSper1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - CatSper1 (extracellular) Antibody AG1315-050
    Western blot analysis of rat testis (lanes 1 and 3) and mouse (lanes 2 and 4) lysates: 1. Anti-CatSper1 (extracellular) antibody (#AG1315), (1:200).
    2. Anti-CatSper1 (extracellular) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q91ZR5
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 78706 Da
Homology Rat, human - 12/14 amino acid residues identical.
Additional Information
Gene ID 225865
Other Names Cation channel sperm-associated protein 1, CatSper1, Catsper1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1 (Accession Q91ZR5). 3rd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte1-3. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis)3,4. Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pHi1,2, which render the sperm chemotaxis-responsive and hyperactive6,7. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust1-3. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm1-7.     CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity7, and is chemotactically responsive to progesterone8. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperβ (2 TM domains), CatSperγ and CatSperδ (1 TM domain each)1, 2. While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits1,3.   CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels; a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel2. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+)3. Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels7.   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse CatSper1. Anti-CatSper1 (extracellular) antibody (#AG1315) can be used in western blot analysis. It has been designed to recognize CatSper1 from mouse, rat and human samples.


References 1. Ren, D. and Xia, J. (2010) Physiology 25, 165. 2. Darszon, A. et al. (2011) Physiol. Rev. 91, 1305. 3. Kirichok, Y. and Lishko, P.V. (2011) Mol. Hum. Reprod. 17, 478. 4. Visconti, P.E. et al. (2011) Asian J. Androl. 13, 395. 5. Reid, A.T. et al. (2011) Asian J. Androl. 13, 88. 6. Yoshida, M. and Yoshida, K. (2011) Mol. Hu. Repro. 17, 457.

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$ 367.00
$ 475.00
Cat# AG1315-050
Availability: 2-3 weeks
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