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Connexin-26 Antibody

Affinity purified polyclonal antibody

  • WB - Connexin-26 Antibody AG1317-050
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes: 1, 2. Anti-Connexin-26 antibody (#AG1317), (1:200).
    3, 4. Anti-Connexin-26 antibody, preincubated with the control peptide antigen.
  • ICC - Connexin-26 Antibody AG1317-050
    Expression of Connexin-26 human HepG2 liver hepatocellular carcinoma cells Immunocytochemical staining of fixed and permeabilized HepG2 liver hepatocellular carcinoma cells. A. Cells were stained with Anti-Connexin-26 antibody (#AG1317), (1:400), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21994
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26451 Da
Homology Mouse - identical; human - 12/13 amino acid residues identical.
Additional Information
Gene ID 394266
Other Names Gap junction beta-2 protein, Connexin-26, Cx26, Gjb2, Cxn-26
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994). Intracellular, cytoplasmic loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. Mutations in the gene encoding for Connexin-26 protein are associated with deafness7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-26. Anti-Connexin-26 antibody (#AG1317) can be used in western blot analysis and immunocytochemistry applications and was designed to recognize Connexin-26 from human, rat and mouse samples.


References 1. Goodenough, D.A. and Paul, D.L. (2011) Cold Spring Harb. Persp. Biol. 2009, 1. 2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423. 3. Chew, S.S.L. et al. (2010) Exp. Neurol. 225, 250. 4. Trexter, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836. 5. Hu, X. et al. (2006) Biophys. J. 90, 140. 6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555. 7. Martin, P.E. et al. (1999) Hum. Mol. Genet. 8, 2369.

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$ 475.00
$ 575.00
Cat# AG1317-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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