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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Connexin-32 Antibody   

Connexin-32 Antibody

Affinity purified polyclonal antibody

  • WB - Connexin-32 Antibody AG1318-050
    Western blot analysis of rat liver lysate: 1.  Anti-Connexin-32 antibody (#AG1318), (1:200).
    2.  Anti-Connexin-32 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08033
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 32004 Da
Homology Human, mouse-identical.
Additional Information
Gene ID 29584
Other Names Gap junction beta-1 protein, Connexin-32, Cx32, GAP junction 28 kDa liver protein, Gjb1, Cxn-32
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 247-260 of rat Connexin-32 (Accession P08033). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. A mutation in the gene encoding Connexin-32 is associated with and is the cause for Charcot–Marie–Tooth disease (CMT), a form of demyelinating disease characterized by muscular weakness, affecting more males than females7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-32. Anti-Connexin-32 antibody (#AG1318) can be used in western blot analysis and was designed to recognize Connexin-32 from human, rat and mouse samples.


References 1. Goodenough, D.A. and Paul, D.L. (2011) Cold Spring Harb. Persp. Biol. 2009, 1. 2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423. 3. Chew, S.S.L. et al. (2010) Exp. Neurol. 225, 250. 4. Trexter, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836. 5. Hu, X. et al. (2006) Biophys. J. 90, 140. 6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555. 7. Kleopa, K.A. (2011) J. Neurosci. 31, 17753.

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$ 475.00
$ 575.00
Cat# AG1318-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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