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Affinity purified polyclonal antibody
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P08033 |
| Reactivity | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 32004 Da |
| Homology | Human, mouse-identical. |
| Gene ID | 29584 |
|---|---|
| Other Names | Gap junction beta-1 protein, Connexin-32, Cx32, GAP junction 28 kDa liver protein, Gjb1, Cxn-32 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 247-260 of rat Connexin-32 (Accession P08033). Intracellular, C-terminus. |
| Dilution | WB~~1:200-1:2000 |
| Peptide Confirmation | Confirmed by mass-spectrography and amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Buffer After Reconstitution | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl DDW. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. A mutation in the gene encoding Connexin-32 is associated with and is the cause for Charcot–Marie–Tooth disease (CMT), a form of demyelinating disease characterized by muscular weakness, affecting more males than females7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-32. Anti-Connexin-32 antibody (#AG1318) can be used in western blot analysis and was designed to recognize Connexin-32 from human, rat and mouse samples.
References
References 1. Goodenough, D.A. and Paul, D.L. (2011) Cold Spring Harb. Persp. Biol. 2009, 1. 2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423. 3. Chew, S.S.L. et al. (2010) Exp. Neurol. 225, 250. 4. Trexter, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836. 5. Hu, X. et al. (2006) Biophys. J. 90, 140. 6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555. 7. Kleopa, K.A. (2011) J. Neurosci. 31, 17753.
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