|Calculated MW||40233 Da|
|Homology||Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.|
|Other Names||Gap junction alpha-5 protein, Connexin-40, Cx40, Gja5, Cxn-40|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GHRFPQGYHSDKR, corresponding to amino acid residues 328-340 of rat Connexin-40 (Accession P28234). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Connexins are transmembrane proteins, which oligomerize into hexameres adjusting in hemi-channels (connexons). The docking of connexons between two neighboring cells results in the formation of intercellular channels, which are permeable to ions and small molecules1. These channels, which are also known as gap junctions, are characterized by distinct electrophysiological properties. Various physiological and pathological conditions regulate the opening and closing of the channels2. Several genetic disorders have been identified in which specific connexin genes are mutated3. Connexin 40 (Cx40) gene consists two exons, a small untranslated exon, exon 1, and an uninterrupted coding region located in an exon 24. In human and mouse species, Cx40 is expressed in various tissues and cells, such as choriocarcinoma cell lines, tonsil lymphocytes and cardiomyocytes5. The expression of the Cx40 gene in cardiomyocytes suggested that in the adult heart, Cx40 involved in the propagation of the electrical impulses from the atria to the ventricles. It was supported by the finding that Cx40 knockout mice developed cardiac conduction defects which matched the pattern of expression of the gene6. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-40. Anti-Connexin-40 antibody (#AG1319) can be used in western blot analysis, and has been designed to recognize Connexin-40 from mouse, rat and human samples.
References 1. Beyer, E.C. et al. 1993. Int. Rev. Cytol. 137C, 1. 2. Evans, W.H. et al. 2002. Mol. Membr. Biol. 19, 121. 3. Kelsell, D.P. et al. 2001. Trends Cell Biol. 11, 2. 4. Bierhuizen, M.F. et al. 2000. Research 46, 511. 5. Dupont, E. et al. 2001. J. Mol. Cell Cardiol. 33, 359. 6. Miquerol, L. et al. 2003. Novartis Found Symp. 250, 80.
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