|Calculated MW||43008 Da|
|Homology||Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.|
|Other Names||Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6. Abgent is pleased to offer a highly specific antibody directed against an epitope of human Connexin-43. Anti-Connexin-43 antibody (#AG1320) can be used in western blot analysis, immunohistochemistry and immunocytochemistry applications and was designed to recognize Connexin-43 from human, rat and mouse samples.
References 1. Goodenough, D.A. and Paul, D.L. (2011) Cold Spring Harb. Persp. Biol. 2009, 1. 2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423. 3. Chew, S.S.L. et al. (2010) Exp. Neurol. 225, 250. 4. Trexter, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836. 5. Hu, X. et al. (2006) Biophys. J. 90, 140. 6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 155.
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