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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRPC6 (extracellular) Antibody   

TRPC6 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPC6 (extracellular) Antibody AG1321-025
    Western blot analysis of rat brain lysates: 1. Anti-TRPC6 (extracellular) antibody (#AG1321), (1:200).
    2. Anti-TRPC6 (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - TRPC6 (extracellular) Antibody AG1321-025
    Expression of TRPC6 in rat PC12 cells Immunocytochemical staining of intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC6 (extracellular) antibody (#AG1321), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining with DAPI as the counterstain. C. Merged images of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61143
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 106681 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 22068
Other Names Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Transient receptor protein 6, TRP-6, Trpc6, Trp6, Trrp6
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl,  50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Rat pheochromocytoma cell line (PC12), (1:50). See immunocytochemistry.


References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Winn. M.P. et al. (2005) Science 308, 1801. 7. Reiser, J. et al. (2005) Nat. Genet. 37, 739.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1321-025
(40 western blots)
Availability: 2-3 weeks
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