|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||106681 Da|
|Homology||Mouse, human - identical.|
|Other Names||Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Transient receptor protein 6, TRP-6, Trpc6, Trp6, Trrp6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Rat pheochromocytoma cell line (PC12), (1:50). See immunocytochemistry.
References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Winn. M.P. et al. (2005) Science 308, 1801. 7. Reiser, J. et al. (2005) Nat. Genet. 37, 739.
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