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TRPC4 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPC4 (extracellular) Antibody AG1322-050
    Western blot analysis of rat and mouse brain lysates: 1. Anti-TRPC4 (extracellular) antibody (#AG1322), (1:200).
    2. Anti-TRPC4 (extracellular) antibody, preincubated with the control antigen.
  • ICC - TRPC4 (extracellular) Antibody AG1322-050
    Expression of TRPC4 in rat PC12 cells Immunocytochemical staining of intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPC4 (extracellular) antibody (#AG1322), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
  • IHC - TRPC4 (extracellular) Antibody AG1322-050
    Expression of TRPC4 in rat brain Immunohistochemical staining of rat third ventricle region using Anti-TRPC4 (extracellular) antibody (#AG1322). A. TRPC4 staining (red) is detected along the wall of the third ventricle (horizontal arrow) and astrocytic fibers extending from the wall (vertical arrow). B. Nucleus staining using DAPI as the counterstain (blue). C. Merge images of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O35119
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 111848 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 84494
Other Names Short transient receptor potential channel 4, Trp4, TrpC4, Capacitative calcium entry channel 1, CCE1, Trpc4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4. The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. the TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.


References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.

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$ 475.00
$ 575.00
Cat# AG1322-050
(40 western blots)
Availability: 2-3 weeks
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