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>   home   >   Products   >   Primary Antibodies   >   CaV alpha2delta2 (extracellular) Antibody   

CaV alpha2delta2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - CaV alpha2delta2 (extracellular) Antibody AG1326-025
    Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), Mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates:

    1-5. Anti-Cavα2δ2 (extracellular) antibody (#AG1326), (1:200).
    6-10. Anti-Cavα2δ2 (extracellular) antibody, preincubated  with the control  peptide antigen.

  • IHC - CaV alpha2delta2 (extracellular) Antibody AG1326-025
    Expression of Cavα2δ2 in rat cerebellum Immunohistochemical staining rat cerebellum using Anti-Cavα2δ2 (extracellular) antibody (#AG1326). A. Cavα2δ2 (green) appears in the soma of purkinje cells (arrow) and in the molecular layer (M). B. Pre-incubation of the antibody with the control peptide antigen blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8CFG6
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 130776 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 300992
Other Names Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2, Voltage-dependent calcium channel subunit alpha-2-2, Voltage-dependent calcium channel subunit delta-2, Cacna2d2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity (C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of ֲ ratֲ  Cav־±2־´2 (Accession Q8CFG6). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Xenopus oocytes expressing α2δ2 subunit (see Edvardson, S. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Voltage-gated Ca2+ channels (CaV), enable the passage of Ca2+ ions in a voltage dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel1. CaV1 and CaV2 channels are high-voltage activated (HVA) CaV channels. The a1 subunit of these channels normally interacts and associates with α2δ subunit, a membrane anchored protein and CaVβ, a cytosolic protein1. Four α2δ subunits have been cloned to date: α2δ1- 4. This subunit originates from a single gene. The corresponding protein is modified by post-translational cleavage yielding a α2 subunit which is disulfide bonded to the δ subunit2. All α2δ subunits are GPI- (glycosylphosphatidylinositol) anchored proteins3. The role of this subunit is important for the membrane trafficking of the α1 subunit, and also has a role in influencing the biophysical properties of the channel1. α2δ can be expressed as various splice variants and expressed in a tissue specific manner. α2δ2 can be detected in the brain, heart, lung, spleen and liver4. Gabapentin and pregabalin are two commonly used anti-epileptic drugs. They act on CaV channels via the α2δ1 and α2δ2 subunits by disturbing their membrane trafficking, thereby decreasing Ca2+ currents.


References 1. Bauer, C.S. et al. (2010) Curr. Opin. Neurobiol. 20, 563. 2. Jay, S.D. et al. (1991) J. Biol. Chem. 266, 3287. 3. Davies, A. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 1654. 4. Klugbauer, N. et al. (2003) J. Bioenerg. Biomembr. 35, 639.

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Cat# AG1326-025
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