|Reactivity||Human, Mouse, Rat|
|Calculated MW||130776 Da|
|Homology||Mouse, human - identical.|
|Other Names||Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2, Voltage-dependent calcium channel subunit alpha-2-2, Voltage-dependent calcium channel subunit delta-2, Cacna2d2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||(C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of ֲ ratֲ Cav־±2־´2 (Accession Q8CFG6). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Xenopus oocytes expressing α2δ2 subunit (see Edvardson, S. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated Ca2+ channels (CaV), enable the passage of Ca2+ ions in a voltage dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel1. CaV1 and CaV2 channels are high-voltage activated (HVA) CaV channels. The a1 subunit of these channels normally interacts and associates with α2δ subunit, a membrane anchored protein and CaVβ, a cytosolic protein1. Four α2δ subunits have been cloned to date: α2δ1- 4. This subunit originates from a single gene. The corresponding protein is modified by post-translational cleavage yielding a α2 subunit which is disulfide bonded to the δ subunit2. All α2δ subunits are GPI- (glycosylphosphatidylinositol) anchored proteins3. The role of this subunit is important for the membrane trafficking of the α1 subunit, and also has a role in influencing the biophysical properties of the channel1. α2δ can be expressed as various splice variants and expressed in a tissue specific manner. α2δ2 can be detected in the brain, heart, lung, spleen and liver4. Gabapentin and pregabalin are two commonly used anti-epileptic drugs. They act on CaV channels via the α2δ1 and α2δ2 subunits by disturbing their membrane trafficking, thereby decreasing Ca2+ currents.
References 1. Bauer, C.S. et al. (2010) Curr. Opin. Neurobiol. 20, 563. 2. Jay, S.D. et al. (1991) J. Biol. Chem. 266, 3287. 3. Davies, A. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 1654. 4. Klugbauer, N. et al. (2003) J. Bioenerg. Biomembr. 35, 639.
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