|Reactivity||Human, Mouse, Rat|
|Calculated MW||38264 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Calcium homeostasis modulator protein 1, Protein FAM26C, CALHM1, FAM26C|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human calcium homeostasis modulator protein 1 (Accession Q8IU99). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer’s disease (AD). Indeed Neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1. Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum (ER) although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1. In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2. Abgent is pleased to offer a highly specific antibody directed against an epitope of human calcium homeostasis modulator 1. Anti-Calcium Homeostasis Modulator 1 antibody (#AG1327) can be used in western blot and immunocytochemistry applications. It has been designed to recognize calcium homeostasis modulator 1 from rat, mouse and human samples.
References 1. Green, K.N. and LaFerla, F.M. (2008) Neuron 59, 190. 2. Koppel, J. et al. (2011) Mol. Med. 17, 974. 3. Dreses-Werringloer, U. et al. (2008) Cell 133, 1149.
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