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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Two Pore Calcium Channel Protein 2 (extracellular) Antibody   

Two Pore Calcium Channel Protein 2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Two Pore Calcium Channel Protein 2 (extracellular) Antibody AG1331-025
    Western blot analysis of mouse kidney lysate (lanes 1 and 4), rat lung membrane (lanes 2 and 5) and human embryonic Kidney 293 cell lysate (lanes 3 and 6):
    1-3. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody (#AG1331), (1:200).
    4-6. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Two Pore Calcium Channel Protein 2 (extracellular) Antibody AG1331-025
    Expression of Two pore calcium channel 2 in rat cerebellum
    Immunohistochemical staining of Two pore calcium channel 2 in rat cerebellum using Anti-Two pore calcium channel 2 (extracellular) antibody (#AG1331). A. TPC2  positive cells (green) appear in the granule cell layer (G) of the cerebellum (arrows). B. The extent of the granule layer is demonstrated with the counterstain DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BWC0
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 83595 Da
Homology Mouse, rat, human- 12/13 amino acid residues identical.
Additional Information
Gene ID 233979
Other Names Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, Tpcn2, Tpc2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide KKTLKSIRW(S)LPE(C) , corresponding to amino acid residues 187-199 of mouse Two pore calcium channel protein 2ֲ  with replacement of amino acid 192 with serine (S) (Accession Q8BWC0). 2nd extracellular loop for Two pore calcium channel protein 2 expressed on the plasma membrane.ֲ  Luminal for Two pore calcium channel protein 2 expressed on intracellular organelles.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (Nav) and Ca2+ (Cav) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2. Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4. In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9. As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Two Pore Calcium Channel Protein 2. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody (#AG1331) can be used in western blot analysis, and has been designed to recognize Two Pore Calcium Channel Protein 2 from mouse, rat and human samples.


References 1. Zhu, M.X. et al. (2010) Am. J. Physiol. 298, C430. 2. Ishibashi, K. et al. (2000) Biochem. Biophys. Res. Commun. 270, 370. 3. Galione, A. et al. (2009) Pflugers Arch. 458, 869. 4. Berridge, G. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 11. 5. Lee, H.C. (2005) J. Biol. Chem. 280, 33693. 6. Churchill, G.C. et al. (2002) Cell 111, 703. 7. Brailoiu, E. et al. (2009) J. Cell. Biol. 186, 201. 8. Calcraft, P.J. et al. (2009) Nature 459, 596. 9. Ruas, M. et al. (2010) Curr. Biol. 20, 703.

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Cat# AG1331-025
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