|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||77423 Da|
|Homology||Rat, mouse, bovine, canis - identical.|
|Other Names||Stromal interaction molecule 1, STIM1, GOK|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586).ֲ ֲ STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Luminal, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.45 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||10 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest. Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+ -binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs. Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human STIM1. Anti-STIM1 (extracellular) antibody (#AG1335) can be used in western blot, immunocytochemical, immunohistochemical, and indirect flow cytometry applications and has been designed to recognize STIM1 from human, rat, mouse, bovine, and canis samples.
References 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. 5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123. 6. Yuan, J.P. et al. (2007) Nat. Cell Biol. 9, 636. 7. Muik, M. et al. (2008) J. Biol. Chem. Jan 10 [Epub ahead of print]. 8. Li, Z. et al (2007) J. Biol. Chem. 282, 29448. 9. Hewavitharana, T. et al. (2007) Cell Calcium 42, 173.
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