|Calculated MW||33062 Da|
|Homology||Rat - identical.|
|Other Names||Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, Orai1, Cracm1, Tmem142a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 ofֲ mouse Orai1 (Accession Q8BWG9 ). 2nd extracellular loop.Will not recognize human Orai1.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.65 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation1,2. Intracellular Ca2+ levels are controlled by either the influx of Ca2+ through the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores which gained much attention. Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8. Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Orai1. Anti-Orai1 (extracellular) antibody (#AG1336) can be used in western blot and indirect flow cytometry applications, and has been designed to recognize Orai1 channel from mouse and rat samples.
References 1. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. 2. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794. 5. Luik, R.M. et al. (2006) J.Cell. Biol.174, 815. 6. Wu, M.M. et al. (2006) J.Cell. Biol.174, 803. 7. Hong H.L. et al. (2007) J. Biol. Chem. 282, 9105. 8. Luik,R.M and Lewis, R.S (2007) Trends Mol.Med. 13, 103. 9. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548.
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