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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Orai2 Antibody   

Orai2 Antibody

Affinity purified polyclonal antibody

  • WB - Orai2 Antibody AG1337-050
    Western blot analysis of HL-60 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
    1, 2. Anti-Orai2 antibody (#AG1337), (1:200).
    3, 4. Anti-Orai2 antibody, preincubated with the control peptide antigen.
  • IHC - Orai2 Antibody AG1337-050
    Expression of Orai2 in rat lung Immunohistochemical staining of paraffin-embedded rat lung sections using Anti-Orai2 antibody (#AG1337), (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue) and alveoli walls (red). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BH10
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28199 Da
Homology Human, rat- identical.
Additional Information
Gene ID 269717
Other Names Protein orai-2, Transmembrane protein 142B, Orai2, Tmem142b
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest. Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5 Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.6 Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7 Abgent is pleased to offer a new antibody directed against an intracellular epitope of the mouse Orai2 channel. Anti-Orai2 antibody (#AG1337) can be used in western blot and immunohistochemical applications and should recognize Orai2 from human, rat, and mouse samples.


References 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. 5. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548. 6. Mercer, J.C. et al. (2006) J. Biol. Chem. 281, 24979. 7. Gwack, Y. et al. (2007) J. Biol. Chem. 282, 16232.

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$ 367.00
$ 475.00
Cat# AG1337-050
Availability: 2-3 weeks
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