|Calculated MW||28199 Da|
|Homology||Human, rat- identical.|
|Other Names||Protein orai-2, Transmembrane protein 142B, Orai2, Tmem142b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest. Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5 Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.6 Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7 Abgent is pleased to offer a new antibody directed against an intracellular epitope of the mouse Orai2 channel. Anti-Orai2 antibody (#AG1337) can be used in western blot and immunohistochemical applications and should recognize Orai2 from human, rat, and mouse samples.
References 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. 5. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548. 6. Mercer, J.C. et al. (2006) J. Biol. Chem. 281, 24979. 7. Gwack, Y. et al. (2007) J. Biol. Chem. 282, 16232.
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