|Calculated MW||127685 Da|
|Homology||Canis- identical; bovine - 12/13 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8, LTRPC6, TRPP8|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop.Will not recognize mouse or rat Orai1.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.65 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1 The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.1 The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.2 TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5 Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human TRPM8. Anti-TRPM8 (extracellular) antibody (#ACC-049) can be used in western blot analysis, immunoprecipitation, immunohistochemical and immunocytochemical applications. It recognizes TRPM8 from human, rat and mouse samples.
References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Brauchi, S. et al. (2004) Proc. Natl. Acad. Sci. 101, 15494. 3. McKemy, D.D. et al. (2002) Nature 416, 52. 4. Peier, A.M. et al. (2002) Cell 108, 705. 5. Dragoni, I. et al. (2006) J.Biol.Chem. 281, 37353. 6. Zhang, L. and Barritt, G.J. (2006) Endocr. Relat. Cancer 13, 27.
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