|Reactivity||Human, Mouse, Rat|
|Calculated MW||109691 Da|
|Homology||Mouse- 12/14 amino acid residues identical; rat- 11/14 amino acid residues identical.|
|Other Names||Polycystin-2, Autosomal dominant polycystic kidney disease type II protein, Polycystic kidney disease 2 protein, Polycystwin, R48321, PKD2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of ֲ human TRPP1 (Accession Q13563). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4. TRPP1 (polycystin-2, PC2, PKD2) belongs to the TRPP subfamily of TRP channels along with TRPP3 and TRPP5 proteins, and forms non-selective cation channels with different permeability to various divalent cations5,6. The cellular localization of TRPP1 has been and still is the subject of a lasting debate. In many cell-types, TRPP1 is retained in the endoplasmic reticulum (ER) where it most likely functions as Ca2+ release channel7,8, and with the help of cofactors, TRPP2 reaches the plasma membrane and the cilia8. TRPP1 expression is widespread and is best characterized for its expression in the kidney where it is developmentally regulated9,10. In the kidney, it associates with PKD1 (TRPP2) an eleven transmembrane-spanning protein (which does not belong to the TRP superfamily) to form functional channels11. In addition, TRPP1 is identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD)5,12. Abgent is pleased to offer a highly specific antibody directed against an epitope of human TRPP1. Anti-TRPP1 (PKD2) antibody (#AG1339) can be used in western blot analysis and immunocytochemistry applications and has been designed to recognize TRPP1 from human, rat and mouse samples.
References 1. Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619. 2. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233. 3. Montell, C. (2005) Sci. STKE 2005, re3. 4. Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19. 5. Tsiokas, L. (2009) Am. J. Renal. Physiol. 297, F1. 6. Owsianik, G. et al. (2006) Annu. Rev. Physiol. 68, 685. 7. Koulen, P. et al. (2002) Nat. Cell Biol. 4, 191. 8. Giamarchi, A. et al. (2006) EMBO Rep. 7, 787. 9. Wilson, P.D. (2001) J. Am. Soc. Nephrol. 12, 834. 10. Venkatachalam, K. and Montell, C. (2007) Annu. Rev. Biochem. 76, 387. 11. Hanaoka, K. et al. (2000) Nature 408, 990. 12. Mochizuki, T. et al. (1996) Science 272, 1339.
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