|Reactivity||Human, Mouse, Rat|
|Calculated MW||197571 Da|
|Homology||Rat, mouse - 15/16 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC-3, LTrpC3, Melastatin-2, MLSN2, TRPM3, KIAA1616, LTRPC3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3 (Accession Q9HCF6 ). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4. The TRPM subfamily consists of 8 members divided in to three major groups: TRPM1/3, TRPM4/5, and TRPM6/7. TRPM2 and TRPM8 are not included in any of the groups since they are quite different even with respect to each other. TRPM3 is alternatively spliced into active variants5,6. TRPM3a1 and TRPM3a2 in mouse are monovalent and divalent-selective channels, respectively. This differential selectivity strongly suggests that their cellular role depends on the relative expression of each variant type. In addition, both splice variants display constitutive outwardly rectifying currents. They somewhat resemble TRPM6 and TRPM7 channels such that their currents are inhibited by intracellular Mg2+ 1. TRPM3 is highly expressed in the kidney, and to a lesser extent in the brain, testis, and spinal cord7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human TRPM3. Anti-TRPM3 (extracellular) antibody (#AG1340) can be used in western blot analysis, and has been designed to recognize TRPM6 from mouse, rat and human samples.
References 1. Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619. 2. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233. 3. Montell, C. (2005) Sci. STKE 2005, re3. 4. Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19. 5. Grim, C. et al. (2003) J. Biol. Chem. 278, 21493. 6. Oberwinkler, J. et al. (2005) J. Biol. Chem. 280, 22540. 7. Clapham, D.E. et al. (2005) Pharmacol. Rev. 57, 427.
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