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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRPM8 (extracellular) Antibody   

TRPM8 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPM8 (extracellular) Antibody AG1341-025
    Western blot analysis of prostate carcinoma cell lines; DU145 (lanes 1,3),  LNCaP (lanes 2,4) and mouse-TRPM8 transfected HEK-293 (lanes 5,6) cell lysates : 1,2,5. Anti-TRPM8 (extracellular) antibody (#AG1341), (1:200).
    3,4,6. Anti-TRPM8 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - TRPM8 (extracellular) Antibody AG1341-025
    Expression of TRPM8 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) antibody (#AG1341), (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
  • ICC - TRPM8 (extracellular) Antibody AG1341-025
    Expression of TRPM8 in LNCaP prostate carcinoma cell line
    Immunocytochemical staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody (#AG1341). A. Extracellular staining of intact LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:100), followed by goat anti-rabbit-AlexaFluor-550 (red), (x100). B. Intracellular staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:1000), followed by goat anti-rabbit-AlexaFluor-488 (green), (x100).
  • ICC - TRPM8 (extracellular) Antibody AG1341-025
    Expression of TRPM8 in rat DRG cells
    Immunocytochemical staining of intact living rat root dorsal root ganglion (DRG) cells. A. Intracellular staining of cells with Anti-TRPM8 (extracellular) antibody (#AG1341), (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Extracellular staining of live cells with Anti-TRPM8 (extracellular) antibody (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z2W7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 127685 Da
Homology Canis- identical; bovine- 12/13 amino acid residues identical;rat, mouse- 11/13 amino acid residues identical.
Additional Information
Gene ID 79054
Other Names Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8, LTRPC6, TRPP8
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide SDVDGTTYDFAHC,ֲ corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7).ֲ ֲ 3rd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.1 The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.2 TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5 Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human TRPM8. Anti-TRPM8 (extracellular) antibody (#AG1341) can be used in western blot analysis, immunoprecipitation, immunohistochemical and immunocytochemical applications. It recognizes TRPM8 from human, rat and mouse samples.


References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Brauchi, S. et al. (2004) Proc. Natl. Acad. Sci.  101, 15494. 3. McKemy, D.D. et al. (2002) Nature 416, 52. 4. Peier, A.M. et al. (2002) Cell 108, 705. 5. Dragoni, I. et al. (2006) J.Biol.Chem. 281, 37353. 6. Zhang, L. and Barritt, G.J. (2006) Endocr. Relat. Cancer 13, 27.

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Cat# AG1341-025
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