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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   TRPM5 Antibody   

TRPM5 Antibody

Affinity purified polyclonal antibody

  • WB - TRPM5  Antibody AG1343-025
    Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 cells (lanes 5 and 7) and human LNCaP cell (lanes 6 and 8) lysates:
    1, 3, 5, 6. Anti-TRPM5 antibody (#AG1343), (1:200).
    2, 4, 7, 8. Anti-TRPM5 antibody, preincubated with the control peptide antigen.
  • IHC - TRPM5  Antibody AG1343-025
    Expression of TRPM5 in rat tongue
    Immunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 antibody (#AG1343), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.
  • IHC - TRPM5  Antibody AG1343-025
    Expression of TRPM5 in rat testes
    Immunohistochemical staining of rat epididymis paraffin embedded sections using Anti-TRPM5 antibody (#AG1343), (1:100). TRPM5 is expressed in principal cells within the epididymal epithelium (arrows). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JJH7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 130844 Da
Homology Rat, human - 13/14 amino acid residues identical.
Additional Information
Gene ID 56843
Other Names Transient receptor potential cation channel subfamily M member 5, Long transient receptor potential channel 5, LTrpC-5, LTrpC5, MLSN1- and TRP-related gene 1 protein, Trpm5 {ECO:0000312|MGI:MGI:1861718}
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7).ֲ ֲ Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3.  The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3.  TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.   TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2.   Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.   Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM5. The Anti-TRPM5 antibody (#AG1343) can be used in western blot and immunohistochemical applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.


References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Vazquez, E. and Valverde, M.A. (2006) Semin. Cell Dev. Biol. 17, 607. 3. Kraft, R. and Harteneck, C. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204. 4. Fang, D. and Setaluri, V. (2000) Biochem Biophys Res Commun. 279, 53. 5. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Montell, C. (2005) Sci. STKE.  2005, re3.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1343-025
(40 western blots)
Availability: 2-3 weeks
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