|Reactivity||Human, Mouse, Rat|
|Calculated MW||172202 Da|
|Homology||Rat- 15/16 amino acid residues identical; human- 14/16 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily M member 2, Long transient receptor potential channel 2, LTrpC-2, LTrpC2, Transient receptor potential channel 7, TrpC7, Trpm2, Ltrpc2, Trpc7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120ֲ of mouse TRPM2 (Accession Q91YD4).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. The C-terminal regions of three TRPM members (TRPM2, TRPM 6 and TRPM 7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4. TRPM2 is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM2. The Anti-TRPM2 antibody (#AG1344) can be used in western blot and immunohistochemical applications. It has been designed to recognize TRPM2 from mouse, rat and human samples.
References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Kraft, R. and Harteneck, C. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204. 3. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690. 4. Montell, C. (2005) Sci. STKE. 2005, re3. 5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. USA. 106, 7239.
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