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>   home   >   Products   >   Primary Antibodies   >   TRPV2 (extracellular) Antibody   

TRPV2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPV2 (extracellular) Antibody AG1345-025
    Western blot analysis of ND7/23 cell line membrane (1,3), RBL lysates (2,4) and rat brain membrane (5,6):
    1,2,5. Anti-TRPV2 (extracellular) antibody (#AG1345), (1:200).
    3,4,6. Anti-TRPV2 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - TRPV2 (extracellular) Antibody AG1345-025
    Immunoprecipitation of rat basophilic leukemia (RBL) cell lysate:
    1. RBL lysate.
    2. Lysate immunoprecipitated with Anti-TRPV2 (extracellular) antibody (#AG1345), (6 mg).
    3. Lysate immunoprecipitated with pre-immune rabbit serum.
    The upper arrow indicates TRPV2 while the lower arrow indicates the IgG heavy chain.
    Western blot analysis was performed with Anti-TRPV2 (extracellular) antibody.
  • IHC - TRPV2 (extracellular) Antibody AG1345-025
    Expression of TRPV2 in mouse DRG Immunohistochemical staining of TRPV2 in mouse dorsal root ganglion (DRG) using Anti-TRPV2 (extracellular) antibody (#AG1345).  A. TRPV2 (green) appears in patches along the perimeter of the DRG (arrows). B) Neurons containing neurofilament 200 (red) are scattered in the DRG, also in patches (arrows). C. A merge of the two panels shows that the spatial distribution of neurofilament 200 and TRPV2 expression overlaps. However, DRGs showing robust expression of neurofilament 200 do not contain TRPV2.
  • ICC - TRPV2 (extracellular) Antibody AG1345-025
    Expression of TRPV2 in RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells with Anti-TRPV2 (extracellular) antibody (#AG1345), (1:100), followed by goat anti-rabbit-AlexaFluor-550 secondary antibody (red), (x100).
  • FC - TRPV2 (extracellular) Antibody AG1345-025
    Indirect flow cytometry analysis of intact living RBL cells: Black Unstained cells.
    ___ Cells + Anti-TRPV2 (extracellular) antibody (#AG1345).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9WUD2
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 86706 Da
Homology Mouse - 15/16 amino acid residues identical.
Additional Information
Gene ID 29465
Other Names Transient receptor potential cation channel subfamily V member 2, TrpV2, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, Vanilloid receptor-like protein 1, VRL-1, Trpv2, Sac2b, Vrl1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2).ֲ 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3 According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7 Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation.8-10 The TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway.8 TRPV2 channel is expressed in DRG neurons, different brain region and non-neuronal tissues such as spleen, lung and, intestine and in component of the immune system.5,11-12 Abgent is pleased to release two novel antibodies to both intracellular TRPV2 and extracellular TRPV2, Anti-TRPV2 (#ACC-032) and Anti-TRPV2 (extracellular) (#AG1345) respectively.


References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Tominaga, M. and Caterina, M.J. (2004) J.Neurobiol. 61, 3. 7. Voets, T. et al. (2004) Nature 430, 748. 8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233. 9. Muraki, K. et al. (2003) Circ. Res. 93, 829. 10. Shimosato, G. et al. (2005) Pain 119, 225. 11. Saunders, C.I. et al. (2007) Mol. Immunol. 44, 1429. 12. Caterina, M.J. et al. (1999) Nature 398, 436.

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Cat# AG1345-025
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