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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   TRPA1 (extracellular) Antibody   

TRPA1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPA1 (extracellular) Antibody AG1346-025
    Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates:
    1,3,4. Anti-TRPA1 (extracellular) antibody (#AG1346), (1:200).
    2,5,6. Anti-TRPA1 (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - TRPA1 (extracellular) Antibody AG1346-025
    Expression of TRPA1 in rat PC12 cells Immunocytochemical staining of intact living rat PC12 cells. A. Extracellular staining of cells using Anti-TRPA1 (extracellular) antibody (#AG1346), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
  • IP - TRPA1 (extracellular) Antibody AG1346-025
    Immunoprecipitation of PC-12 lysates: 1. PC-12 lysates.
    2. PC-12 lysates + Anti-TRPA1 (extracellular) antibody (#AG1346) (6.5 µg) + protein A beads.
    3. Anti-TRPA1 (extracellular) antibody + protein A beads.
  • IHC - TRPA1 (extracellular) Antibody AG1346-025
    Expression of TRPA1 in mouse DRG Immunohistochemical staining of mouse dorsal root ganglion (DRG) frozen sections using Anti-TRPA1 (extracellular) antibody (#AG1346). A. TRPA1 (green) was distributed in patches (arrows). B. Neurons containing neurofilament 200 (red) also were distributed in patches. C. Confocal merge of TRPA1 and  neurofilament 200 demonstrate partial overlap of these patches (arrows).
  • IHC - TRPA1 (extracellular) Antibody AG1346-025
    Expression of TRPA1 in rat DRG Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPA1 (extracellular) antibody (#AG1346) (1:200). A. TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75762
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 127501 Da
Homology Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.
Additional Information
Gene ID 8989
Other Names Transient receptor potential cation channel subfamily A member 1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, TRPA1, ANKTM1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)NSTGIINETSDHSE,ֲ corresponding to amino acidֲ residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Application Details Western blot analysis (WB): - Human urethra (1:2000) (see Gratzke, C. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Human urethra (1:2000) (see Gratzke, C. et al. (2009) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

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The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli.1 Originally it was thought that TRPA channels sensed painfully cold temperatures2, but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations by cold3-5 and perhaps stretch.6 In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil. TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial.1 TRPA1 has similar structure as all TRP ion channel members, six transmembrane domains, intracellular N-and C-terminus. However, its N-terminus domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor.6,7 TRPA1 is expressed in nociceptive neurons expressing TRPV1 as well and might serve as a marker for poly-modal nociceptors.8


References 1. Hill, K. and Schaefer, M. (2007) J. Biol. Chem. 282, 7145 2. Story, G.M. et al. (2003) Cell 112, 819. 3. Bandell, M. et al. (2004) Neuron 41, 849. 4. Jordt, S.E. et al. (2004) Nature 427, 260. 5. Zurborg, S. et al. (2007) Nat. Neurosci. 10, 277. 6. Corey, D.P. et al. (2004) Nature 432, 723. 7. Sotomayor, M. et al. (2005) Structure 13, 669. 8. Clapham, D.E. (2002) Science 295, 2228.

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Cat# AG1346-025
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