|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||127501 Da|
|Homology||Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily A member 1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, TRPA1, ANKTM1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NSTGIINETSDHSE,ֲ corresponding to amino acidֲ residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Application Details||Western blot analysis (WB): - Human urethra (1:2000) (see Gratzke, C. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Human urethra (1:2000) (see Gratzke, C. et al. (2009) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli.1 Originally it was thought that TRPA channels sensed painfully cold temperatures2, but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations by cold3-5 and perhaps stretch.6 In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil. TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial.1 TRPA1 has similar structure as all TRP ion channel members, six transmembrane domains, intracellular N-and C-terminus. However, its N-terminus domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor.6,7 TRPA1 is expressed in nociceptive neurons expressing TRPV1 as well and might serve as a marker for poly-modal nociceptors.8
References 1. Hill, K. and Schaefer, M. (2007) J. Biol. Chem. 282, 7145 2. Story, G.M. et al. (2003) Cell 112, 819. 3. Bandell, M. et al. (2004) Neuron 41, 849. 4. Jordt, S.E. et al. (2004) Nature 427, 260. 5. Zurborg, S. et al. (2007) Nat. Neurosci. 10, 277. 6. Corey, D.P. et al. (2004) Nature 432, 723. 7. Sotomayor, M. et al. (2005) Structure 13, 669. 8. Clapham, D.E. (2002) Science 295, 2228.
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