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TRPV5 Antibody

Affinity purified polyclonal antibody

  • WB - TRPV5 Antibody AG1348-050
    Western blot analysis of rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates:
    1,2. Anti-TRPV5 antibody (#AG1348), (1:200).
    3,4. Anti-TRPV5 antibody, preincubated with the control peptide antigen.
  • IHC - TRPV5 Antibody AG1348-050
    Expression of TRPV5 in rat kidney
    Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 antibody (#AG1348), (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.
  • ICC - TRPV5 Antibody AG1348-050
    Expression of TRPV5 in PC3 cells Immunocytochemical staining of paraformaldehyde-fixed PC-3 cells with Anti-TRPV5 antibody (#AG1348), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Same as in (A) but pre-treated with TRPV5 peptide antigen (1:20). Hoechst 33342 is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NDW5
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Homology Rat - 11/15 amino acid residues identical; rabbit - 13/15 amino acid residues identical.
Additional Information
Gene ID 56302
Other Names TRPV5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5 (Accession Q8NDW5). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in kidney. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope in the C-terminus of the human TRPV5. Anti-TRPV5 antibody (#AG1348) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize TRPV5 from human and rat samples.


References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Hoenderop, J.G.J. et al. (2003) Pflugers Arch 446, 304. 7. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.

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$ 475.00
$ 575.00
Cat# AG1348-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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