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TRPV4 Antibody

Affinity purified polyclonal antibody

  • WB - TRPV4 Antibody AG1349-025
    Western blot analysis of ND7/23 cell line lysate:
    1. Anti-TRPV4 antibody (#AG1349), (1:200).
    2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
  • WB - TRPV4 Antibody AG1349-025
    Western blot analysis of rat brain lysates: 1. Anti-TRPV4 antibody (#AG1349), (1:200).
    2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
  • IHC - TRPV4 Antibody AG1349-025
    Expression of TRPV4 in rat DRG Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 antibody (#AG1349). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
  • ICC - TRPV4 Antibody AG1349-025
    Expression of TRPV4 in rat DRG primary culture Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.
    A, D. Staining using Anti-TRPV4 antibody (#AG1349), (1:500), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
    B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.
    C. Merged image of panels A and B.
    F. Merged image of panels D and E.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9ERZ8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 98010 Da
Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.
Additional Information
Gene ID 66026
Other Names Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Trpv4, Vroac
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).ֲ ֲ Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Application Details Immunoprecipitation (IP): - Rat and mouse brain lysates (10 µg/500 mg lysate) (see Benfenati, V. et al. (2011) in Product Citations).   Immunocytochemistry (IC): - Mouse distal nephrons (1:1000) (see Mamenko, M. et al. (2013) in Product Citations).   Indirect flow cytometry (IFC): - Human corneal epithelial cells (HCEC) (1:200) (see Pan, Z. et al. (2008) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.6


References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.

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$ 375.00
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$ 575.00
Cat# AG1349-025
(40 western blots)
Availability: 2-3 weeks
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