|Calculated MW||130531 Da|
|Homology||Rat - 12/13 amino acid residues identical.|
|Other Names||Short transient receptor potential channel 2, TrpC2, Transient receptor protein 2, TRP-2, mTrp2, Trpc2, Trp2, Trrp2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KNRSGVRMFKDGD, corresponding to amino acid residues 12-24 of mouse (Accession Q9R244 ). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.9 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
TRPC (transient receptor potential cation channel) channels are the closest mammalian homologs of Drosophila TRP and TRP-like channels. TRPCs are rather nonselective Ca2+ permeable cation channels and affect cell functions through their ability to mediate Ca2+ entry into cells and their action to collapse the plasma membrane potentials1. The mammalian genome codes for seven TRPCs of which TRPC2 is the largest with the most restricted pattern of expression and has several alternatively spliced variant2. Expressed in model cells, TRPC2 mediates both receptor- and store depletion-triggered Ca2+ 3. In human, TPRC2 is a pseudogene3. Physiological roles for TRPC2 have been studied in mature sperm and the vomeronasal sensory system. In sperm, TRPC2 is activated by the sperm’s interaction with the oocyte’s zona pellucida, leading to entry of Ca2+ and activation of the acrosome reaction4. In the vomeronasal sensory organ (VNO), TRPC2 was found to constitute the transduction channel activated through signaling cascade initiated by the interaction of pheromones with V1R and V2R G protein-coupled receptors on the dendrites of the sensory neurons5. TRPC2-deficient mice display abnormal mating behavior, consistent with a role for this channel in pheromone signaling6.
References 1. Birnbaumer, L. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 15195. 2. Vannier, B. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 2060. 3. Liman, E.R. et al. (1999). Proc Natl Acad Sci USA 96, 5791. 4. Jungnickel, M.K. et al. (2001) Nat. Cell Biol. 3, 499. 5. Liman, E.R. et al. (1996) J. Neurosci. 16, 4625. 6. Stowers, L. et al. (2002) Science 295, 1493.
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