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>   home   >   Products   >   Primary Antibodies   >   CaV3.2 Antibody   

CaV3.2 Antibody

Affinity purified polyclonal antibody

  • WB - CaV3.2 Antibody AG1352-025
    Western blot analysis of rat DRG lysates:
    1. Anti-Cav3.2 antibody (#AG1352), (1:200).
    2. Anti-Cav3.2 antibody, preincubated with the control peptide antigen.
  • IHC - CaV3.2 Antibody AG1352-025
    Expression of Cav3.2 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Cav3.2 antibody (#AG1352), (1:100). A. Cav3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of Cav3.2 adjacent to Purkinje cells.
  • IHC - CaV3.2 Antibody AG1352-025
    Expression of CaV3.2 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections with Anti-CaV3.2 antibody (#AG1352), (1:50). Staining is specific for DRG. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342  is used as the counterstain.
  • ICC - CaV3.2 Antibody AG1352-025
    Expression of Cav3.2 in rat DRG primary culture Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.
    A, D. Immunocytochemical staining using Anti-Cav3.2 antibody (#AG1352), (1:200), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody.
    B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342.
    C. Merged image of panels A and B.
    F. Merged image of panels D and E.
    A-C: x20
    D-F: x100
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9EQ60
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 261113 Da
Homology Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
Additional Information
Gene ID 114862
Other Names Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav32, Cacna1h
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of ratֲ  Cav3.2 (Accession number Q9EQ60ֲ ).ֲ ֲ Intracellular loop between domainsֲ D1 and D2.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Mouse lung and brain lysate (1:200) (see Wan, J. et al. (2013) in Product Citations). - Human CaV3.2 transfected in HEK-293 cells (1:100) (see Markandeya, Y.S. et al. (2011) in Product Citations). Immunoprecipitation (IP): - Rat brain lysates (5 μg) (see Weiss, N. et al. (2012) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.  T-type channels are also known to participate in the pacemaker activities of heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as Cav3.2 (α1H), Cav3.1 (α1G) and Cav3.3 (α1I).1-3  The Cav3.2  (α1H) channel is widely expressed in various tissues, brain, heart, liver and testis. Involvement of Cav3.2 Ca2+ channels in several pathologies has been described. Overexpression of the Cav3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the Cav3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in Cav3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice. Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the Cav3.2 Ca2+channels. This might indicate a possible role for Cav3.2 in nociception.7 


References 1. Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707. 2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678. 3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530. 4. Gackière, F. et al. (2008) J. Biol. Chem. 283, 10162. 5. Khosravani, H. et al. (2004) J. Biol. Chem. 279, 9681. 6. Chen, C.C. et al. (2003) Science 302,1416. 7. Bourinet, E. et al. (2005) EMBO J.  24, 315.

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Cat# AG1352-025
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