|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||261113 Da|
|Homology||Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.|
|Other Names||Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav32, Cacna1h|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of ratֲ Cav3.2 (Accession number Q9EQ60ֲ ).ֲ ֲ Intracellular loop between domainsֲ D1 and D2.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse lung and brain lysate (1:200) (see Wan, J. et al. (2013) in Product Citations). - Human CaV3.2 transfected in HEK-293 cells (1:100) (see Markandeya, Y.S. et al. (2011) in Product Citations). Immunoprecipitation (IP): - Rat brain lysates (5 μg) (see Weiss, N. et al. (2012) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as Cav3.2 (α1H), Cav3.1 (α1G) and Cav3.3 (α1I).1-3 The Cav3.2 (α1H) channel is widely expressed in various tissues, brain, heart, liver and testis. Involvement of Cav3.2 Ca2+ channels in several pathologies has been described. Overexpression of the Cav3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the Cav3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in Cav3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6 Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the Cav3.2 Ca2+channels. This might indicate a possible role for Cav3.2 in nociception.7
References 1. Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707. 2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678. 3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530. 4. Gackière, F. et al. (2008) J. Biol. Chem. 283, 10162. 5. Khosravani, H. et al. (2004) J. Biol. Chem. 279, 9681. 6. Chen, C.C. et al. (2003) Science 302,1416. 7. Bourinet, E. et al. (2005) EMBO J. 24, 315.
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