|Reactivity||Human, Mouse, Rat|
|Calculated MW||250407 Da|
|Homology||Mouse- identical; human - 20/22 amino acid residues identical.|
|Other Names||Voltage-dependent T-type calcium channel subunit alpha-1G, Voltage-gated calcium channel subunit alpha Cav31, Cacna1g|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CaV3.1 (Accession O54898). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse HL-1 cells (see Nguyen, N. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Rat heart sections (1:20) (see Atkinson, A.J. et al. (2013) in Product Citations). - Human myometrium (1:200) (see Blanks, A.M. et al. (2007) in Product Citations). Immunocytochemistry (IC): - Human myometrium cells (1:200) (see Blanks, A.M. et al. (2007) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the Cav channel family.2-3 The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pace making activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms. CaV3.1 (α1G) and CaV3.2 (α1H) are widely distributed whereas the expression of CaV3.3 (α1I) is restricted to the central nervous system.2 CaV3.1 and CaV3.2 are also expressed in the kidney, but little is known about their physiological role there.
References 1. Serrano, J.R. et al. (1999) J. Gen. Physiol. 114, 185. 2. Perez-Reyes, E. (2003) Physiol. Rev. 83, 117. 3. Wain, H.M. et al. (2002) Nucleic Acids Researc 30, 169. 4. Andreasen, D. et al. (2000) Am.J.Physiol. 279, F997.
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